找生物-分享有趣、实用的生物技巧!
做平板对于各位童鞋来说,应该是非常easy的事情,但是也会有些小问题导致实验失败,以下讲述些小技巧可以保证每次都能够制备出完美的平板-没有气泡、或气雾等。
1. Make up the medium according to the recipe, then add the desired amount of agar (normally about 1% w/v) and stir
If you autoclave without stirring, with the argarose still floating on top of the liquid, you get an agarose cake in the medium. Interesting, but useless
2. Autoclave for 25 m...
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